跪求分析化学大神解答 要解答过程 谢谢 以反相健合相色谱法测定某药物及其代谢物,柱长15c

1.a=1/[0.5*(0.52+0.58)]=1.818

2.0.95/a*15cm=7.84

L=1m,tRA=5.80min,tRB=6.60min,WA=0.78min,WB=0.82min,t0=1.10min

(1)u=L/ t0=100cm/1.10min=90.9cm/min

(2)к=tR′/ t0=(6.60-1.10)/1.10=5.00

(3)R=2(tRB-tRA)/(WA+WB)=2(6.60-5.80)/(0.78+0.82)=1

(4)neff =16(tRA′/WA)2=16×〔(5.80-1.10)/0.78〕2=581

Heff =L/neff=1/581=1.72mm

(5)R1/R2= √L1 /√L2 1/1.5= √1m/√L2 L2=2.25

(6)tRB=2.25×6.60min=14.85min